| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221449 |
| Chromosome: | chromosome 2 |
| Location: | 2848274 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094700 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCTATTGTGCTCATGTATCATAGTGC |
| Internal bar code: | GTGATTTTTCGTGCGTAGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1080 |
| LEAP-Seq percent confirming: | 99.4718 |
| LEAP-Seq n confirming: | 20716 |
| LEAP-Seq n nonconfirming: | 110 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTTTGAGCCACCTTGC |
| Suggested primer 2: | ATCCAAGTCGTCATCGCTCT |