| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221461 |
| Chromosome: | chromosome_9 |
| Location: | 4479735 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g395769 | FAP177 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCTGTGTTGCTGTACGCCTCCCCCCCCC |
| Internal bar code: | GCCACAATACCAGACGGGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 99.5285 |
| LEAP-Seq n confirming: | 2322 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGACAAGCTCAAGGAGG |
| Suggested primer 2: | TTTTTCAGGTTGCGGATTTC |