| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221463 |
| Chromosome: | chromosome_7 |
| Location: | 2496180 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g329500 | SUB1 | Secreted protease and protease inhibitor | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AACACCGATACAAGCGATGCAAACAATGTC |
| Internal bar code: | GTCCCCGACTAGGTCGGGACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 858 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGTAGCGCAAACCAGAG |
| Suggested primer 2: | ATTGCACCTGGAATGACACA |