| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221493 |
| Chromosome: | chromosome 5 |
| Location: | 3359178 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240750 | TEF17 | Predicted protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGTCAGGTCCAGCCCAGGGTCCTGGC |
| Internal bar code: | CACATTCTATGACTGGGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 909 |
| LEAP-Seq percent confirming: | 99.1057 |
| LEAP-Seq n confirming: | 5541 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGATCCGAGCATGTACGA |
| Suggested primer 2: | ACACTCAACCGTGGGAAGAC |