| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221497 |
| Chromosome: | chromosome 17 |
| Location: | 5344157 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736400 | SCC3 | Cohesin complex subunit; (1 of 1) K06671 - cohesin complex subunit SA-1/2 (STAG1_2, SCC3, IRR1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAGTGAGTGGACTAAAGCGTAGCAGTC |
| Internal bar code: | GCGCCAGCTGATGCGGATAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 96.2006 |
| LEAP-Seq n confirming: | 1899 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTGCAGCAGTGTGCTCT |
| Suggested primer 2: | ACACTATCCGCCTGGATCAC |