Insertion junction: LMJ.RY0402.221508_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.221508
Chromosome:chromosome 17
Location:2609391
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g717050 (1 of 16) IPR003439//IPR003593//IPR013525//IPR027417 - ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTTCGTTTTTTACACCACGCACACACGC
Internal bar code:TTGGGTCCCGACTTTCATAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:496
LEAP-Seq percent confirming:77.6263
LEAP-Seq n confirming:2505
LEAP-Seq n nonconfirming:722
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGCTCATGTGTGTGCGTA
Suggested primer 2:CATAAAGCACGTCGTCCTGA