| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221532 |
| Chromosome: | chromosome 5 |
| Location: | 701759 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230700 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAAGGCCGAGATGACATTGGTTGTCTG |
| Internal bar code: | GGGATCCCATTCGAAGCGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 813 |
| LEAP-Seq percent confirming: | 94.88 |
| LEAP-Seq n confirming: | 8580 |
| LEAP-Seq n nonconfirming: | 463 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAACCCGTACTTGGAGT |
| Suggested primer 2: | TTCAGCTTTGAATGAATGCG |