| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221555 |
| Chromosome: | chromosome 17 |
| Location: | 4647921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g733300 | (1 of 1) PTHR24361:SF417 - NIK-RELATED PROTEIN KINASE | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGTCTGTAGTGACATTTTTGGATGGGG |
| Internal bar code: | GTAGGAACGGGTGCAGGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 92.1519 |
| LEAP-Seq n confirming: | 364 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTACGCTCGCTGTCCTT |
| Suggested primer 2: | TCTGTTTGAACTCGCCTGTG |