| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221561 |
| Chromosome: | chromosome 3 |
| Location: | 4841629 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179000 | RAA16 | component psaA trans-splicing sub complex II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATAAGAGCTTCAGCCATGCTGCCGTGC |
| Internal bar code: | CTTCGGCTCTCCCCCCCGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 97.9068 |
| LEAP-Seq n confirming: | 4537 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGTGCTGCCAAGTCGTA |
| Suggested primer 2: | GGGTAGCTCGCTATTCGTTG |