| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221648 |
| Chromosome: | chromosome_10 |
| Location: | 903868 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g424250 | FAP90 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATCAAGCACAAGCAAATAGCAATCGCGTG |
| Internal bar code: | GACCGAGGATATAAGGTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 933 |
| LEAP-Seq percent confirming: | 99.4691 |
| LEAP-Seq n confirming: | 6932 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTCTGTTTGGAACCGCAC |
| Suggested primer 2: | CAGTCGTGCTTCTTGGTGAA |