| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221659 |
| Chromosome: | chromosome 10 |
| Location: | 4077843 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449600 | SPR1 | Sepiapterin reductase; (1 of 1) 1.1.1.153 - Sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTCGAGGAGAACCGCCGCGCGGGCCGC |
| Internal bar code: | TGCCGGTAACCGCGGCCATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 127 |
| LEAP-Seq percent confirming: | 84.8 |
| LEAP-Seq n confirming: | 424 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGTCTCTGGCCAACAT |
| Suggested primer 2: | GCCAGAGTGCATGACTGGTA |