| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221661 |
| Chromosome: | chromosome 14 |
| Location: | 389088 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610566 | (1 of 3) PF02209 - Villin headpiece domain (VHP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCCGCAAGTCCTGGGCATTGTGGTTAC |
| Internal bar code: | GTGATTGGACGTCGCAGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 58.9986 |
| LEAP-Seq n confirming: | 872 |
| LEAP-Seq n nonconfirming: | 606 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATGGTATATGGTGGGCG |
| Suggested primer 2: | CCCAGAAACCACTCCTGTGT |