| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221706 |
| Chromosome: | chromosome 17 |
| Location: | 3734387 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g727400 | HRP8 | Hydroxyproline-rich glycoprotein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGTTGGCTACTTGCTCATTGCCTCTGT |
| Internal bar code: | CAGCTAGGTCCGGGGGAAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 90.5093 |
| LEAP-Seq n confirming: | 3643 |
| LEAP-Seq n nonconfirming: | 382 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCGAAGTGTTCTGTTGC |
| Suggested primer 2: | CGGACGAGCAAAGTGTGATA |