| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221724 |
| Chromosome: | chromosome 11 |
| Location: | 1836765 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGACCTTCCGTGCTTGACAGGCACAACG |
| Internal bar code: | ATGCTCACGCCGACTGACGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 99.6037 |
| LEAP-Seq n confirming: | 4273 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACCTGGAGTCATTCAG |
| Suggested primer 2: | CTTTTCCTTGCAGCATGTCA |