| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221728 |
| Chromosome: | chromosome 7 |
| Location: | 436445 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315326 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGACAGCTGCACGGAGCGCATCGGGCC |
| Internal bar code: | CCTGCGGGACCATCGCTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGAATGCTGACACGAT |
| Suggested primer 2: | CCTCACCTCCATGATCCACT |