| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221746 |
| Chromosome: | chromosome 5 |
| Location: | 3268176 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240225 | (1 of 1) IPR008754//IPR024079 - Peptidase M43, pregnancy-associated plasma-A // Metallopeptidase, catalytic domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACATTCCTACACTTCTCTGTGCCATCT |
| Internal bar code: | TCGATTGGCCTGGCGCGATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 99.5484 |
| LEAP-Seq n confirming: | 6833 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCCACATACTGAACCC |
| Suggested primer 2: | CTCGCTTCGTCTCTCTTGCT |