| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221839 |
| Chromosome: | chromosome 17 |
| Location: | 1489917 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707050 | UAF5,SPL12 | U2 snRNP auxiliary factor associated protein; (1 of 1) K13093 - HIV Tat-specific factor 1 (HTATSF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTATGGAAACCCTCTTGCTTTGACTTTC |
| Internal bar code: | ATTCTTACCCTACGCGCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.6586 |
| LEAP-Seq n confirming: | 3211 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCGTGACAACTGGCTGA |
| Suggested primer 2: | ATTCTCACAACGCTGCCTCT |