Insertion junction: LMJ.RY0402.221855_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.221855
Chromosome:chromosome 10
Location:2343741
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g434950 (1 of 1) PF05010 - Transforming acidic coiled-coil-containing protein (TACC) (TACC) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTAACATACAGTCGGAGATTGACAAGGT
Internal bar code:GAGCCTGTCCGGTAAATTGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1022
LEAP-Seq percent confirming:99.2106
LEAP-Seq n confirming:2388
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCACACACAGGCTTACAG
Suggested primer 2:GGGACAGCTTCTTCTTCACG