| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221865 |
| Chromosome: | chromosome 3 |
| Location: | 4663501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177500 | (1 of 2) K17279 - receptor expression-enhancing protein 5/6 (REEP5_6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCTGAAGTGTCCGAGCCACGACGTTGT |
| Internal bar code: | TAGGCCCAGGCTACGGTCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 98.8201 |
| LEAP-Seq n confirming: | 670 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCTTGGCTTAGAGTTG |
| Suggested primer 2: | ACGCTGGGTATGTCACAACA |