| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221867 |
| Chromosome: | chromosome 12 |
| Location: | 3061773 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500450 | TPD1 | (1 of 2) PTHR12121:SF37 - 2',5'-PHOSPHODIESTERASE 12; 2'%252C5'-phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGAAGCACCAGCGCGCGACGGTAGGCG |
| Internal bar code: | TTAGCCCGATCTCGTGTTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.5871 |
| LEAP-Seq n confirming: | 12541 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCTTTGCAACCACACAC |
| Suggested primer 2: | GCGTAGACAGGAACAGGAGC |