| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221876 |
| Chromosome: | chromosome 11 |
| Location: | 3044791 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g478800 | (1 of 1) PTHR31027//PTHR31027:SF2 - FAMILY NOT NAMED // PROTEIN F18H3.4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCCGGACTGGGACACTACACCGGCCAG |
| Internal bar code: | GTAGCTCTTCATATAATCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 99.68 |
| LEAP-Seq n confirming: | 3115 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGAGCTTCACGTCCAC |
| Suggested primer 2: | CAGAATGAACAGAGGGGGAA |