| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221878 |
| Chromosome: | chromosome 11 |
| Location: | 3187300 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g479383 | (1 of 1) IPR000104//IPR013122 - Antifreeze protein, type I // Polycystin cation channel, PKD1/PKD2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACGGAGGGAGACGTGTACAGCATATGT |
| Internal bar code: | CGTTTGGACATCGACTTTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCCTGGTCCTTAGTCAT |
| Suggested primer 2: | CACTGGTCCTCCTACCGTGT |