Insertion junction: LMJ.RY0402.221893_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.221893
Chromosome:chromosome 14
Location:3982542
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g633300 (1 of 1) IPR003582//IPR006626//IPR009030 - ShKT domain // Parallel beta-helix repeat // Insulin-like growth factor binding protein, N-terminal intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCGTCGGCCCCTACCCGCCACGCGCTG
Internal bar code:TTGCACGAGGTGTGCTATTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:246
LEAP-Seq percent confirming:98.6477
LEAP-Seq n confirming:12255
LEAP-Seq n nonconfirming:168
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGGTTATGACCGTGCCT
Suggested primer 2:CCACCACACACACACAGACA