| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221924 |
| Chromosome: | chromosome 3 |
| Location: | 595222 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145587 | CPLD1 | Conserved in the Plant Lineage and Diatoms; (1 of 4) PF04134 - Protein of unknown function, DUF393 (DUF393) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGACGCCATGGATGGTCTCCATTGCCTG |
| Internal bar code: | CGACGCTACGGGTGCTGCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 99.6783 |
| LEAP-Seq n confirming: | 13942 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCAACACAATGGGAATC |
| Suggested primer 2: | CGTCTTGCACCGTAGTCAGA |