| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221937 |
| Chromosome: | chromosome 14 |
| Location: | 2499313 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625150 | PGM8 | (1 of 1) PTHR23029:SF26 - PROTEIN Y18H1A.4-RELATED; Phosphoglycerate mutase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCATATTGTTACCCCAACTTGTCCCTT |
| Internal bar code: | TTTCTGTACATGCAAAATGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATGCTGCTACTGCTGCT |
| Suggested primer 2: | CATGCACAAAACCACCAGTC |