| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221938 |
| Chromosome: | chromosome 10 |
| Location: | 3662825 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445850 | MAE14 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF); MATE efflux family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACAGGAAGCAAGTCAGCGGTGGATGCC |
| Internal bar code: | TCCCTCCCGTACAAGCTGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 399 |
| LEAP-Seq percent confirming: | 99.4494 |
| LEAP-Seq n confirming: | 6322 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCATCTGACAGGACGAA |
| Suggested primer 2: | TGTGTGTGTGCATGATTTGG |