| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221964 |
| Chromosome: | chromosome 14 |
| Location: | 1778574 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619850 | ELG36 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT; Exostosin-like membrane protein, a heparan sulfate glycosyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGGACACACGCAGCAGCCCCCCGCCAC |
| Internal bar code: | GCGCCCGTTCGCCTCGATTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGGCCGTTAAGGTACAG |
| Suggested primer 2: | ATGAATTGGTGTAGCTCGGG |