| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.221975 |
| Chromosome: | chromosome 1 |
| Location: | 4923854 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034100 | (1 of 2) K01408 - insulysin (IDE, ide) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAGAAGGTTTGCCCCCGCTTCGCAACC |
| Internal bar code: | GAGCGGGGCGGAGCCGCTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 99.0133 |
| LEAP-Seq n confirming: | 3211 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGCTTGCAAACAAGTCC |
| Suggested primer 2: | CCACTCACTAGGAGAAGGCG |