Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.221989 |
Chromosome: | chromosome_3 |
Location: | 8784275 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g205697 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCTCATTGGGGATGCTAAAATCCCCCTTA |
Internal bar code: | GAACTCGTGAAATATGTGTACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 236 |
LEAP-Seq percent confirming: | 98.2206 |
LEAP-Seq n confirming: | 276 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGAGAAGGGTGAAAGCAGC |
Suggested primer 2: | TGCACTTCTCTGCACCAATC |