| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221996 |
| Chromosome: | chromosome 12 |
| Location: | 5923375 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534200 | (1 of 1) PTHR24365//PTHR24365:SF381 - TOLL-LIKE RECEPTOR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGCTTGAGCAGGAGGGCAGGTGCCGGG |
| Internal bar code: | GGCGGTAATTGTTGTGTCACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 119 |
| LEAP-Seq percent confirming: | 99.7625 |
| LEAP-Seq n confirming: | 420 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCATCCGAGTACAGCC |
| Suggested primer 2: | GCCTCCATTCACAGAAGCAT |