Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.222043 |
Chromosome: | chromosome_6 |
Location: | 3762985 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278157 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGATTGCAATTTCGGGTTGCCAGTCGTCAA |
Internal bar code: | CGTGTCAAGCGACCCGGTAGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 964 |
LEAP-Seq percent confirming: | 98.2545 |
LEAP-Seq n confirming: | 66365 |
LEAP-Seq n nonconfirming: | 1179 |
LEAP-Seq n unique pos: | 188 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCGACTAGCTAAGGGTGTC |
Suggested primer 2: | AGGTGGAGCTGGTGAGAAGA |