Insertion junction: LMJ.RY0402.222043_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.222043
Chromosome:chromosome 6
Location:3762986
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278157 (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTTATCATCCGTGGCTTGCAATGCCCC
Internal bar code:GTATTTTCCCATATTGTGTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:157
LEAP-Seq percent confirming:99.3908
LEAP-Seq n confirming:4731
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGACTAGCTAAGGGTGTC
Suggested primer 2:AGGTGGAGCTGGTGAGAAGA