| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222060 |
| Chromosome: | chromosome 13 |
| Location: | 3944232 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590800 | HTA21,HTA37 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCCAAGTCCACAAGACCGAGCGCAAGG |
| Internal bar code: | CTTGACATTGACTCTAGTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1024 |
| LEAP-Seq percent confirming: | 99.177 |
| LEAP-Seq n confirming: | 8074 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGAAGGGCAAGTACGC |
| Suggested primer 2: | CTAGAGAGGGACAGCATCGG |