| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222066 |
| Chromosome: | chromosome 3 |
| Location: | 7969442 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201950 | (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTAGTGGCCCCTTCTCGTCCTGTGCTGA |
| Internal bar code: | TAACAGTCTTACGAGTCTGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 99.7417 |
| LEAP-Seq n confirming: | 7722 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGACTCTGTTCTTGAAGG |
| Suggested primer 2: | CAGACCCATGTTGTGACCTG |