Insertion junction: LMJ.RY0402.222070_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.222070
Chromosome:chromosome 7
Location:2331880
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g328000 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCCTGCTCAGAGACCGTCGCCGCCGCTG
Internal bar code:ACGACCGGGTCATAGTGTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:913
LEAP-Seq percent confirming:98.2951
LEAP-Seq n confirming:5189
LEAP-Seq n nonconfirming:90
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCATGGTCTTGTAGTCAG
Suggested primer 2:GTTTGCAACTTGCGCACTAA