| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222073 |
| Chromosome: | chromosome_3 |
| Location: | 8095566 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g200950 | FAP273 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGTAAGGTACTAGCTATACCCAAATGTAA |
| Internal bar code: | GGGACTGGCGTATCACACCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 84.7222 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCATAGAGGCAGGAGTCGC |
| Suggested primer 2: | GCAACCGTTTCAACTTCCAT |