| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222099 |
| Chromosome: | chromosome 10 |
| Location: | 583530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421650 | paralog of LCI36; (1 of 4) K15382 - solute carrier family 50 (sugar transporter) (SLC50A, SWEET) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACGTAACGAACAAGCCGCGTCCGCACCT |
| Internal bar code: | ACAATACGCGCCCCATTCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.4916 |
| LEAP-Seq n confirming: | 47752 |
| LEAP-Seq n nonconfirming: | 244 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTATCTTCATGGTGGGTG |
| Suggested primer 2: | CGTTGCAGAACCAGATGTTG |