| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222137 |
| Chromosome: | chromosome 12 |
| Location: | 9708427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557078 | (1 of 4) 1.14.17.1 - Dopamine beta-monooxygenase / Dopamine beta-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCCATGCATGCGTGCTGGAAGTGTGAC |
| Internal bar code: | CTCCTGGTAGCACCCGCGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 70.4082 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTACCTGTGCCGACACTT |
| Suggested primer 2: | GCGTATGTTTGGGAGTTCGT |