| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222142 |
| Chromosome: | chromosome 9 |
| Location: | 365732 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405050 | ATPVD2,ATPvD2 | Vacuolar ATP synthase subunit D | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCAGCTGCGCGGCTGCTGACCTGCAGG |
| Internal bar code: | CGATTCGTGGCCTCATGTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.5849 |
| LEAP-Seq n confirming: | 5758 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATAGGCTGGTGGTGGTG |
| Suggested primer 2: | GGACGGCAGCACTACCTAAG |