| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222191 |
| Chromosome: | chromosome 10 |
| Location: | 936717 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424600 | (1 of 1) PF14771 - Domain of unknown function (DUF4476) (DUF4476) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGACGAAGTCGTAAGCGAGTGAGCGG |
| Internal bar code: | CTCAATAGCAACTCCCGGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 99.2377 |
| LEAP-Seq n confirming: | 13409 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATTGCTATGCCCTTTCC |
| Suggested primer 2: | AGTTTAGTTTGGGCTCGGGT |