| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222215 |
| Chromosome: | chromosome 12 |
| Location: | 3027255 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500750 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGAGCAACCCTCTCCGCTTGCCCTTGTT |
| Internal bar code: | GCGCCGGCCAATCCCCCCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 98.9742 |
| LEAP-Seq n confirming: | 9552 |
| LEAP-Seq n nonconfirming: | 99 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATTGACTTCAGCGACCC |
| Suggested primer 2: | CCCTAGGCCTTGTGCTCATA |