Insertion junction: LMJ.RY0402.222221_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.222221
Chromosome:chromosome 7
Location:3712405
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g337650 PDC1 Mitochondrial pyruvate dehydrogenase complex, E1 component, alpha subunit; (1 of 1) PTHR11516//PTHR11516:SF40 - PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR // PDHA1 PROTEIN 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTCAGCGTGTCGGTCCTCATTCCGGGCG
Internal bar code:GGTGGGTGTCCTCCTTCCGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:99.8244
LEAP-Seq n confirming:3410
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CTATGCCGAAGAGCCAGAAC
Suggested primer 2:CTTAAACAGCGAGGACGGAG