| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.222221 |
| Chromosome: | chromosome_7 |
| Location: | 3712405 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g337650 | PDC1 | Mitochondrial pyruvate dehydrogenase complex, E1 component, alph | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATTCAGCGTGTCGGTCCTCATTCCGGGCG |
| Internal bar code: | GGTGGGTGTCCTCCTTCCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 99.8244 |
| LEAP-Seq n confirming: | 3410 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGCCGAAGAGCCAGAAC |
| Suggested primer 2: | CTTAAACAGCGAGGACGGAG |