| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222230 |
| Chromosome: | chromosome 11 |
| Location: | 1584534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467759 | PIC1,PIC1-2 | (1 of 2) PTHR34548:SF2 - PROTEIN TIC 21, CHLOROPLASTIC; Similar to AtTic21/PIC1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCCAGGACACGCGTGTCAAACCCCCGT |
| Internal bar code: | GATCTACCGGGACCGCACATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 99.6583 |
| LEAP-Seq n confirming: | 4958 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTGGGGGATAGCACTGT |
| Suggested primer 2: | TCGTTGCCACTCAAGTTACG |