| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222238 |
| Chromosome: | chromosome 12 |
| Location: | 8417327 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548651 | (1 of 8) 2.3.1.75 - Long-chain-alcohol O-fatty-acyltransferase / Wax synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTAGCGGCAATGGCAACGGCAGCGGCG |
| Internal bar code: | CTCTGGTGCGGCACGTCGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 96.1629 |
| LEAP-Seq n confirming: | 11829 |
| LEAP-Seq n nonconfirming: | 472 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGAGCAACCTAAGCCG |
| Suggested primer 2: | GGTGTTGTTGTTGATGCTGG |