| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222251 |
| Chromosome: | chromosome_14 |
| Location: | 792143 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613050 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGCTCTACCTGTCCTATGGCGGCGCCAAC |
| Internal bar code: | AAGAAGCATCTTCGCGCCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.2478 |
| LEAP-Seq n confirming: | 10819 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAATCAGGCCTCTTGCAG |
| Suggested primer 2: | CCCTAAAACGTCACATGGCT |