Insertion junction: LMJ.RY0402.222251_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.222251
Chromosome:chromosome 14
Location:792143
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g613050 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTCTACCTGTCCTATGGCGGCGCCAAC
Internal bar code:AAGAAGCATCTTCGCGCCGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:985
LEAP-Seq percent confirming:99.2478
LEAP-Seq n confirming:10819
LEAP-Seq n nonconfirming:82
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CCTAATCAGGCCTCTTGCAG
Suggested primer 2:CCCTAAAACGTCACATGGCT