| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222256 |
| Chromosome: | chromosome_2 |
| Location: | 5220837 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g105950 | FAP174 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCTCGTGTGTCGACTTGACTCTCAAAAT |
| Internal bar code: | TGTTCGATGGCCCATGAAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 99.4143 |
| LEAP-Seq n confirming: | 8487 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAAGCATTTTGCAATCC |
| Suggested primer 2: | GCGCCTACAGAGACTTGGAC |