| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222281 |
| Chromosome: | chromosome 16 |
| Location: | 610409 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692150 | GT90-29,GT90F29 | (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 29 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGTGGCGGCGTACGTAGTACGGCAGC |
| Internal bar code: | TCGAGCGACGTACGGTATTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 98.992 |
| LEAP-Seq n confirming: | 9919 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCGCTCAATAGCCTGAT |
| Suggested primer 2: | GCACACATACCTGTGCATCC |