| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.222308 |
| Chromosome: | chromosome_9 |
| Location: | 6867284 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g410050 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGTCTTGGTGCTGGTGCGTCATCCGTAA |
| Internal bar code: | TGTTCAGAGCTCAGTAGAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 999 |
| LEAP-Seq percent confirming: | 96.7216 |
| LEAP-Seq n confirming: | 10031 |
| LEAP-Seq n nonconfirming: | 340 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTTGAGAGTGAGCACAA |
| Suggested primer 2: | TGACGATACGCAGCTTGTTC |