| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.222309 |
| Chromosome: | chromosome 1 |
| Location: | 2648015 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015500 | CGL91 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED; Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTATTTACCACTCCTCACCCGTATCGCC |
| Internal bar code: | TTGGCGGTGCAATTCTAAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.6814 |
| LEAP-Seq n confirming: | 3442 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCTTCCACCCTGTCTT |
| Suggested primer 2: | GACAGACGGATGATGGTGTG |