Insertion junction: LMJ.RY0402.222315_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.222315
Chromosome:chromosome 2
Location:6153720
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g113550 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAGCAGCGCGCCTCGGCGCGAGGTCCGA
Internal bar code:CTATCGCTGCAACCGGGAAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:851
LEAP-Seq percent confirming:99.0852
LEAP-Seq n confirming:1408
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTGGGCACGTACGATAG
Suggested primer 2:CGGGTCGTACTGAAGGTTGT